Аутори:
1. Ivan Djuricic, Машински факултет Универзитета у Београду, Serbia
2. Jovana Šakota-Rosić, Машински факултет Универзитета у Београду, Serbia
3. Ivana Stanković (Mileusnić), Машински факултет Универзитета у Београду, 4. Jelena Munćan, Машински факултет Универзитета у Београду, Serbia
5. Dušan Šarac, Univeristy of Belgrade, Faculty of Mechanical Engineering, Serbia
6. Boris Kosić, Машински факултет Универзитета у Београду, Serbia
7. Lidija Matija, Машински факултет Универзитета у Београду, Serbia

Апстракт:
Fourier transformed infrared spectroscopy of human serum albumin (HSA) in the region of 650-4000cm-1 was subjected for developing reagentless measurement of HSA. The infrared spectra were subjected to partial least squares (PLS) regression to obtain calibration models predicting the concentrations of the HSA. The calibration in the concentration of 1 - 6.5mg/dl has yielded a correlation coefficient of 0.99 and a standard error of prediction of 0.11mg/dl with latent variable of six. These result suggests that the infrared spectra of aqueous solutions of HSA and its calibration model developed using PLS regression can extract up effectively the information about albumin contained in water. These results provide a base for development of reagentless method of measurement of HSA in human serum and also in vivo HSA measurement.

Кључне речи:
human serum albumi,FTIR-ATR,PLS regression

Тематска област:
СИМПОЗИЈУМ Ц - Вода

Датум пријаве сажетка:
14.06.2015.

Конференцијa:
Contemporary Materials 2015 - Савремени Материјали

Сажетак рада